1. Purification before culturing can be accomplished by CsCl density gradient centrifugation. 2. Optionally, the final lysate is subjected to further purification by CsCl density gradient . 3. Linderstorm-Lang, in 1937, discovered that density gradient tubes could be used for density measurements. 4. :The extent of a density gradient is almost immeasurable even under conditions favoring it. 5. The tube contains an insert that prevents the mixing of the density gradient medium and blood. 6. :: : : There's no surface tension because there's no surface, just a gradual density gradient . 7. The last term includes temperature and density gradients of the background distribution function, which drive the perturbation. 8. Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. 9. Heavy liquids are often used for determination of density in mineralogy, for density gradient centrifugation and for separating mixtures. 10. Surface heat and freshwater fluxes create global density gradients that drive the thermohaline circulation part of large-scale ocean circulation.